site stats

Pylgrna-u3

TīmeklispYLsgRNA-OsU6a/LacZ. 价格： 1200元. 联系方式： I47-825O-882O. 相关技术服务： 质粒构建 基因合成 质粒大提. 立刻购买. Tīmeklis2024. gada 14. janv. · of the four rice U3 and U6 promoters but retained the USE and ... 0.2 U of high-fidelity DNA polymerase KOD FX, and pYLgRNA-mOsU# plasmids [24] (~20 ng each) as templates, for 25 cycles (95 °C ...

Method for creating long-grain fragrant rice germplasm resources

Tīmeklis2015. gada 14. apr. · 刘耀光多靶点pCRISPR载体(单子叶和双子叶植物用)使用方法2015-4-14.doc,PAGE \* MERGEFORMAT 18 CRISPR/Cas9-based genome editing technology A robust CRISPR/Cas9 vector system for multiplex targeting of genomic sites in monocot and dicot plants 亚热带农业生物资源保护与利用国家重点实验室 华 … Tīmeklis2024. gada 15. febr. · 1.5 杜梨子叶浸染及遗传转化. 杜梨子叶再生参照林静 [18]的方法，遗传转化参照庞宏光 [19]的方法，主要操作包括：杜梨种子消毒、子叶诱导愈伤培 … black country museum prices 2022 https://hotel-rimskimost.com

多靶点pcrispr载体(单子叶和双子叶植物用)使用方法2015-4 …

Tīmeklis2015. gada 14. apr. · The U3 and U6 promoters from rice (Os) and Arabidopsis (At) and the sgRNA sequence are separated by the vector backbone, to avoid amplification of … Tīmeklis2024. gada 18. jūn. · 将三对寡链靶点引物90℃变性后冷却至室温完成退火，再将退火后的3个双链靶点片段分别接连到pylgrna-u3、pylgrna-u6a、pylgrna-u6b载体上，并 … black country museum offers

一种提高水稻耐盐性的分子育种方法

Category:CN106191107A - 一种降低水稻籽粒落粒性的分子改良方法

Tags:Pylgrna-u3

Pylgrna-u3

assets.researchsquare.com

TīmeklisThe binary CRISPR/Cas9 vector pYLCRISPR/Cas9-MH and corresponding gRNA vector pYLgRNA-U3 was provided by Y. G. Liu at South China Agricultural University, … TīmeklisCN106191107B CN201610587707.6A CN201610587707A CN106191107B CN 106191107 B CN106191107 B CN 106191107B CN 201610587707 A …

Pylgrna-u3

Did you know?

Tīmeklis本论文发表于中国水稻科学杂志，属于农业相关论文范文材料。仅供大家论文写作参考。 TīmeklisThe method for creating the long-grain fragrant rice germplasm resources comprises the following steps of: directionally editing a rice grain type gene GS9 and a fragrance gene Badh2 by using a CRISPR/Cas9 technology, connecting a target site sequence, a gRNA guide sequence and a U3/U6a promoter sequence through an intermediate vector …

Tīmeklis2024. gada 15. febr. · 1.5 杜梨子叶浸染及遗传转化. 杜梨子叶再生参照林静 [18]的方法，遗传转化参照庞宏光 [19]的方法，主要操作包括：杜梨种子消毒、子叶诱导愈伤培养、农杆菌介导的遗传转化、筛选培养基培养4个步骤。. 阳性植株检测：将在筛选培养基上生长的抗性芽及对照正常 ... Tīmeklis2024. gada 13. apr. · Description - USB 3.0 Gigabit LAN adapter - Plug&Play compact USB 3.0 LAN network adapter: just plug into a USB port and connect it by a patch cord with your network - Half/full duplex 10/100/1000 Mbps operation - Built-in Flash-drive with drivers. Other features -. dimension depth - 30 mm. EAN - 8716309096690.

TīmeklisImage: Illustrated plasmid map in PNG format GenBank File: Plasmid sequence and annotations. Use text editor or plasmid mapping software to view sequence. … Plasmid pYLCRISPR/Cas9Pubi-H from Dr. Yao-Guang Liu's lab contains the insert … Tīmeklis2014. gada 26. okt. · pYLgRNA-U#的BsaI 图谱见第2 最好把BsaI分装成几管冷冻保存。. （推荐使用以下4+5步骤边切边连方法，这个步骤只做参考，如果采用边切边连方 …

Tīmeklis一站式科研服务平台. 文档翻译; 学术分析; 论文查重; 文档转换; 首页; 成为会员; 我要充值

TīmeklisU6/U3基因由III型RNA聚合酶转录，转录起始点是确定的碱基位置。例如，U6为G碱基，U3为A 碱基。 ... ~100 ng pYLgRNA-U#(或其它有Amp R. 基因的质粒)。37℃反 … black country museum voucher codeTīmeklis2024. gada 14. apr. · 用变温循环进行酶切连接约10-15循环: 375 min；105 min，20 min；最后375min。. 此方法简便效率高，阳性克隆率高，推荐优先使用。. 冷冻保存未用完的连接产物，有必要时做下一步扩增用。. 注：也可以用常规的先切后连法〔供参考〕： 取约2-3 在50μl〔30U BsaI〕酶切30 ... galway bypass routeTīmeklisScientia Agricultura Sinica ›› 2024, Vol. 51 ›› Issue (14): 2631-2641. doi: 10.3864/j.issn.0578-1752.2024.14.001 • CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles Next Articles Using CRISPR/Cas9-Mediated Targeted Mutagenesis of qSH1 … galway capital investmentTīmeklis2024. gada 13. apr. · Description - USB 3.0 Gigabit LAN adapter - Plug&Play compact USB 3.0 LAN network adapter: just plug into a USB port and connect it by a patch … black country museum ticket pricesTīmeklisU6/U3基因由III型RNA聚合酶转录，转录起始点是确定的碱基位置。例如，U6为G碱基，U3为A 碱基。 ... ~100 ng pYLgRNA-U#(或其它有Amp R. 基因的质粒)。37℃反应15min 后电泳检查，以未切的相同质粒为对照。 black country museum virtual tourTīmeklisCN113481214A CN202410992639.2A CN202410992639A CN113481214A CN 113481214 A CN113481214 A CN 113481214A CN 202410992639 A … galway camera shop shop streethttp://www.doczj.com/doc/e78747795.html galway camera shop street