Phis1525

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http://www.diva-portal.org/smash/record.jsf?pid=diva2:887011 WebbThe aim of this research was to express the Î²-xylosidase gene in the pHIS1525/ Bacillus megaterium MS941 system. The xyl genewas amplified from pTP510 and... DOAJ is a …

REKOMBINASI GEN PENYANDI - JOURNAL UNAIR - PDF Free …

WebbEkspresi gen penyandi β-xilosidase dalam sistem pHIS1525/ Bacillus megaterium MS941 by: Sri Sumarsih, et al. Published: (2012-02-01) Implication of Polyhistidine, a Novel Apoptosis Inhibitor, in Inhibiting Lipopolysaccharide-Induced Apoptosis in Boar Sperm by: Tianzeng Song, et al. Published: (2024-09-01) WebbThe recombination plasmid pHIS1525 was transformed into Escherichia coli DH5a and the transformant was designated DH5a-pHIS1525-G7. The plasmid from the recombinant … chippewa tall motorcycle boots

Rekombinasi Gen Penyandi -xilosidase asal Geobacillus …

Webbsubsequently cloned into Bacillus megaterium expression vector; pHIS1525.SP lipA and transformed in Bacillus megaterium ATCC®14945. The kerA gene carrying the … WebbFor further optimization of our project, more vectors are constructed but not listed here. Some things should be mentioned about plasmid pHIS1525, pSPAsp-hp and pHBintE are … WebbpHIS1525 Vector for the secretion of recombinant His6-tagged proteins in B. megaterium (Fig. 2); PxylA-SPlipA-MCS-His-STOP, Tc r (B. megaterium); Apr (E. coli) this work … chippewa tea

Holdings: A pathogenic PSEN2 p.His169Asn mutation associated …

Phis1525

Webbmegaterium MS941 rekombinan ditumbuhkan dalam medium LB (+Tc) pada 37° C dengan pengocokan 250 rpm hingga OD 578 nm = 0,3–0,4 dan setelah ditambahkan larutan xilosa 0,5% WebbBoca Scientific vector phis1525 Vector Phis1525, supplied by Boca Scientific, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - …

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WebbIn order to develop a stable strain of Bacillus megaterium for Keratinase production, the Keratinase gene (KerA) of Bacillus lichiniformis ATCC 53757 and SPlipA gene from plasmid pHIS1525.SPlipA (B ... WebbpHIS1525 (Mobitec GmbH, Göttingen, Germany; Table 2). The RBS of P xylA as optimized by Malten et al. (2005)was introduced by PCR amplification from pHIS1525 by miss-matching positions in the reverse primer Pxyl_RBS+ _Gibson_rv, thereby generating P xylA+. The oligonucleotide primers used in this study are listed in Supplementary

Webb28 juni 2024 · Metode Penelitian Semua prosedur molekuler dilakukan sesuai dengan prosedur standar Sambrook and Rusell. Dimulai dari Isolasi dan pemurnian DNA …

WebbThis research showed that the b-xylosidase gene was succesfully sub-cloned in pHIS1525 system and expressed by the recombinant B. megaterium MS941. Theaddition of 0.5% … Webb21 mars 2016 · pHIS1525 Like pMM1525 with additional sequence for C-terminal 6xHis-tag fusion (including stop codon). pSTREP1525 Like pMM1525 with additional sequence for …

WebbAmplikon diinsersikan pada plasmid pHIS1525, yang keduanya telah didigesti dengan enzim restriksi Sac I dan Sph I. Plasmid rekombinan yang dihasilkan ditransformasikan …

http://journal.unair.ac.id/filerPDF/Vol%2013%20No%203%20September%202411-3.pdf grapeheartWebbpHIS1525 merupakan shuttle vector ( FROL % PHJDWHULXP yang mengandung signal peptide yang terdiri atas sekuens penyandi esterase ekstraseluler asal Bacillus sehingga … chippewa tech collegeWebbwith the CPD, by ligation into pHis1525/TcdB digested with 5-BsrGI and 3-BamHI. To generate AGT-TcdB-BoNT/A-Hc, an AgeI site was installed between the TD and RBD of TcdB. The entire RBD of TcdB, consisting of the C-terminally combined repetitive oligopeptides (CROPs, residues 1852–2366), was chippewa tennisWebbpHIS1525. PVT13488 2ug. pHIS1525 Search name. pHIS1525,pHIS1525 vector,pHIS1525 plasmid,pHIS1525 map. pHIS1525 Information. Bacterial Resistance(s): Amp. Screening: … grapehead strain woodwardWebb巨大芽孢杆菌低温保存法：1.简单保存法，将琼脂斜面孢子培养物、菌丝悬浮液以及由麸皮、大米、小米等谷物原料制成的孢子培养物置于4℃冰箱保存,保存时间不超过1～2个月，若将谷物原料制备的孢子瓶抽真空并在棉塞上浸蜡,以隔绝外界空气和水汽,保持时间可达3～4个 … chippewa tennis ann arborWebbDownload dengan 2 jenis enzim restriksi SacI dan SphI, (2) rekombinasi. (ligasi) amplikon dan plasmid pHIS1525 menggunakan enzim ligase (3) transformasi plasmid ... chippewa terraceWebbpHIS1525 Plasmid - maxanim.com ... pHIS1525 grapeheads candy