WebJan 1, 2024 · Inactivated Mycoplasma hyopneumoniae vaccine is used extensively to control M. hyopneumoniae infection worldwide. Quantification techniques are essential in the process of standardizing and validating vaccines. In this study, we developed and optimized an indirect competitive enzyme linked immunosorbent assay (ic-ELISA) for the rapid … WebApr 19, 2024 · The 96-well ELISA plates (Costar, USA) were individually coated with the optimal concentrations of each recombinant protein, and the ideal serum dilutions were determined by checkerboard titration. The plates were coated overnight at 4 °C with recombinant proteins in the coating buffer (0.05 M carbonate buffer, pH 9.8).
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