WebPublished protocols using the promonocytic cell line THP-1 have tended to result in cells that closely resemble classically-activated macrophages, differentiated in IFNγ and LPS. … Web1 day ago · An increase in exposure of β-(1,3)-glucan and chitin on the cell surface was also observed through flow cytometry. Interestingly, we observed a decrease in the phagocytosis rate when the Δcdc50 mutant was co-incubated with THP-1 macrophages. The Δcdc50 mutant also exhibited weakened virulence in nematode survival tests.
Protocol for high-throughput compound screening using flow cytometry …
WebLearn about controls for flow cytometry including isotype controls, Fc blocks, FMO controls, live-dead, unstained controls, compensation controls and biological controls for surface & intracellular staining. ... WebJun 5, 2015 · At 24 hpi, IE2-EYFP positive cells were measured by flow cytometry; (b) THP-1 monocytes were differentiated to THP-1 derived macrophages by the addition of PMA for 48 h followed by infection of the obtained differentiated THP-1 cells with TB40E/IE2-EYFP at an MOI of 0.1. At 24 hpi, IE2-positive cells were detected by flow … chili\u0027s inglewood
Mannose metabolism inhibition sensitizes acute myeloid leukaemia cells …
WebFeb 3, 2024 · Single-cell preparation from different organs, staining of cells with fluorophore-conjugated antibodies, and analysis of the stained cells using flow … WebFeb 22, 2024 · In terms of phenotypic activation markers, measured by flow cytometry, M1 response was similar but the established MDM M2 marker CD163 was undetectable in THP-1 cells. In a beads-based assay, MDM activation did not induce significant changes, whereas M2 activation of THP-1 decreased phagocytic activity compared to M0 and M1. WebCell cycle assay protocols for flow cytometry. Vybrant DyeCycle Violet Stain. Vybrant DyeCycle Green and Orange Stains. Vybrant DyeCycle Ruby Stain. FxCycle Violet Stain. FxCycle Violet Ready Flow Reagent. FxCycle PI/RNase Staining Solution. FxCycle Far Red Stain. CellEvent Senescence Green Flow Cytometry Assay Kit. grace baptist church of yonkers