Earcht-eyfp
WebeArchT-EYFP into vCA1 (Fig. 2, A and B, and fig. S6). We confirmed that the vCA1 neurons speci-fically project to the NAc shell but not to the NAc core, as identified by tyrosine … Webkinase II (CaMKII):eArchT–enhanced yellow fluorescent protein (eYFP) in the deep layers oftheMECinwild-type(WT)micewithbilat-erallyimplantedoptic fibersabovethePFC,cACC, or RSC (Fig. 1, E and J, and fig. S2G). Expres-sion of eArchT-eYFP was abundant in MEC-Va terminals located in each of these regions (Fig. 1, B and I, and fig. S2D).
Earcht-eyfp
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WebSep 9, 2024 · (A) Sagittal sections of MEC and CA1 area. MECIII-Cre mice were bilaterally infected with the Cre specific AAVrh8-hSyn1-DIO-eArchT-eYFP virus and silicone linear probes with an optical fiber were implanted in both areas. (B) Examples of light stimulation during quiet awake when animals were in an open arena. Top panel: Light OFF condition.
WebApr 4, 2024 · Specifically, context A-A (trained context) vs. context A-B (neutral context) for natural recall ratios, ChR2-eYFP vs. eYFP for engram cell activation ratios, and eArchT-mCherry vs. mCherry for ... WebpLenti-CaMKIIa-eArchT 3.0-EYFP was a gift from Karl Deisseroth (Addgene plasmid # 35513 ; http://n2t.net/addgene:35513 ; RRID:Addgene_35513) For your References section: Principles for applying optogenetic tools …
WebNov 18, 2024 · Third, a subset of mice injected with AAV1-DIO-ChR2-EYFP (n = 3) or AAV2/9-DIO-eArchT-EGFP (n = 4) in the OT for optogenetic behavioral experiments was additionally used for anterograde tracing ... WebProvide outreach programs, equipment and facilities, and funding for sailing education, training, and competition so that students may learn the life skills and values important in …
WebMar 18, 2024 · The amygdala is a key structure for fear memory (Davis, 1992, Duvarci and Pare, 2014, Ehrlich et al., 2009, Maren and Fanselow, 1996) and is also involved in fear extinction memory ( Amano et al., 2010, Grewe et al., 2024, Herry et al., 2008 ). However, it is unknown whether the amygdala is the storage site of fear extinction memory and, if so ...
WebIf you are using these viruses for the first time, please obtain a Material Transfer Agreement (MTA) by contacting Dr. Karl Deisseroth [email protected] and Charu Ramakrishnan [email protected]. Please submit a copy of your MTA along with your virus order. high schools in san mateoWebMar 21, 2024 · T o this end, we made AAV-TetO-eArchT-eYFP-WPRE (TetO::eAr chT) [42], expressing an inhibitory. optogenetic actuator activated by green light. Four weeks after injection of TetO::eAr chT in Tph2-tTA. how many cups is 64 ozWebThe AAVrh8-hSyn1-DIO-eArchT-eYFP plasmid was constructed by inserting the eArchT-eYFP gene fragment, which was obtained from a template, pLenti-CaMKIIa-eArchT_3.0eYFP (courtesy of Dr. Karl Deisseroth at Stanford University) high schools in san mateo countyWebMar 21, 2024 · To this end, we made AAV-TetO-eArchT-eYFP-WPRE (TetO::eArchT) , expressing an inhibitory optogenetic actuator activated by green light. Four weeks after injection of TetO::eArchT in Tph2-tTA mice, we tested the effect of photoinhibition of the DRN in the CPP test (Figure 4 A–D). To assess aversive properties, we associated … high schools in san antonioWebJul 21, 2024 · d Expression of eArchT-EYFP in the DRN and median raphe nucleus. Optic fiber was placed into the DRN (scale bar 500 μm). The timeline of the experiment was … how many cups is 60g of oatsWebEYFP (C terminal on insert) Cloning Information Cloning method Restriction Enzyme 5′ cloning site PacI (not destroyed) 3′ cloning site BsrGI (not destroyed) 5′ sequencing … high schools in san marcos caWebThis website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies. how many cups is 60z