Ctcf knockout 293t cell line
WebPopular answers (1) 8th Mar, 2014. Michelle Ozbun. University of New Mexico. As a point of clarification, HEK293 cells are immortalized by Ad 5 E1A and E1B. HEK293T cells also express SV40 large T ... Web1. Almost 50% of cells were alive even after 6ug/mL puromycin treatment. 2. HEK293 became suspension cells after puromycin treatment. Some details: At first, I tried the puromycin concentration (2 ...
Ctcf knockout 293t cell line
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WebDetection of human CTCF by western blot of immunoprecipitates. Samples: Nuclear Extract (1.0 mg per IP reaction; 20% of IP loaded) from 293T cells.Antibodies: Affinity purified rabbit anti-CTCF antibody A300-543A (lot A300-543A-4) used for IP at 6 µg per reaction. CTCF was also immunoprecipitated by rabbit anti-CTCF antibody A300-542A. WebSep 13, 2024 · Here, we analyze the genome-wide effect of CTCF in a zygotic ctcf knockout mutant in zebrafish that allows us to monitor the effect of CTCF loss of function during embryonic development. CTCF ...
WebFeb 23, 2010 · Although CTCF occupies these sites in both erythroid K562 cells and fibroblast 293T cells, the long-range interaction frequencies among the sites are highly cell type specific, revealing a more densely clustered organization in the absence of globin gene activity. Both CTCF and cohesins are required for the cell-type-specific chromatin ... WebDec 1, 2024 · To study the effects of losing CTCF, we decided to generate knockout alleles in the zebrafish using the CRISPR-Cas9 genome editing system. After a detailed …
WebThis disclosure concerns transcription cassettes comprising nucleic acid molecules comprising a nucleotide sequence encoding AP-4 subunits; vectors comprising said transcription cassettes; pharmaceutical compositions comprising said vector; and vectors or compositions for use in the treatment of AP-4-Hereditary Spastic Paraplegia. WebThe resulting cell line is referred to as CTCF-AID hereafter. Adding auxin to the culture medium depleted CTCF to levels that could not be detected by western blot, and washing out ... CTCF prevented subcloning of CTCF-AID cells, recapitulating the full CTCF knockout phenotype in mESCs (Sleutels et al., 2012)(Figure 1D).
WebJun 22, 2024 · CTCF is a key organizer of the 3D genome. Its specialized paralog, BORIS, heterodimerizes with CTCF but is expressed only in male germ cells and in cancer states. Unexpectedly, BORIS-null mice ...
WebApr 26, 2015 · I have problems with HEK293 cell line and its IC50 value. In literature, 200-500 ng/ml is suggested as g418 concentration. To be convinced, I performed kill curve (in 96-well plate, 600-60 and 800 ... citibank singapore address and swift codeWebCTCF-null mice show early embryonic lethality 11, and conditional knockout of CTCF in the developing mouse limb results in extensive cell death 12 . Cohesin is essential for cell proliferation ... citibank singapore breWebHuman CD47 knockout HEK-293T cell line (ab266324) Datasheet SDS Submit a review Submit a question $3,780.00 Product size 1 × 10 6 cells/vial Add to basket Order now … citibank singapore career loginWebJan 11, 2024 · Here the authors provide further support for the role of MyoD in 3D genome architecture in muscle stem cells by comparing MyoD knockout and wild-type mice. ... CTCF, and 1 × 10 7 cells and 5 µg ... diaper rash or yeast infection babyWebThe 293T cell line, originally referred as 293tsA1609neo, is a highly transfectable derivative of human embryonic kidney 293 cells, and contains the SV40 T-antigen. This cell line is competent to replicate vectors carrying the SV40 region of replication. It gives high titers when used to produce retroviruses. diaper rash or yeast infectionWebJul 17, 2024 · Exogenous overexpression of target genes in both general and specific cell types is important for mechanistic studies of gene function. Here, we provide a step-by-step protocol for cell culture, plasmid transfection in HEK293T, and adenoviral infection in C2C12 cells for gene overexpression in vitro, using MG53 as an example. This protocol enables … citibank singapore credit card giroWebFeb 17, 2024 · Also, in this cell line, STAG1 stability on chromatin was significantly reduced following CTCF depletion (Figure 3—figure supplement 3A and B). ... , as we had previously observed in primary Ctcf ‘knockout’ MEFs arrested in G0; see Figure 1A in Busslinger et al. (2024). This indicates that CTCF is also required for cohesin acetylation in ... diaper rash physical exam description