Cisplatin apoptosis induction concentration

Author: zqbg

August undefined, 2024

WebJan 18, 2016 · Cisplatin induces apoptosis in renal tubular cells. (A and B) NRK-52E cells treated with or without (Ctrl) different concentrations of cisplatin (10–50 μmol/L) for 24 h were subjected to phase ... WebApoptosis inducers act on several apoptosis-related proteins to promote apoptotic cell death. Depending on the agent selected and the concentrations used, apoptotic events …

Apoptosis Induction with Combined Use of Cisplatin and Fisetin in ...

WebSep 30, 2024 · Induction of apoptosis and DNA damage response were evaluated in both monotherapy and combination therapy groups. Results: Results demonstrate that … WebCisplatin is the most important and efficacious chemotherapeutic agent for the treatment of advanced gastric cancer. Cisplatin forms inter- and intrastrand crosslinked DNA adducts … oracle dba internship

Frontiers The Molecular Basis and Therapeutic Aspects of …

WebApr 28, 2011 · Cisplatin is a chemotherapeutic drug widely used in the treatment of a variety of cancers, including sarcomas, lymphomas, and some carcinomas such as small … WebApr 14, 2024 · Among the extracellular vesicles, apoptotic bodies (ABs) are only formed during the apoptosis and perform a relevant role in the pathogenesis of different diseases. Recently, it has been demonstrated that ABs from human renal proximal tubular HK-2 cells, either induced by cisplatin or by UV light, can lead to further apoptotic death in … WebJul 5, 2024 · The obtained results from flow cytometry analysis showed that the rate of apoptosis was 4.26% in the cells treated with cisplatin, 38.69% in the cells treated with cisplatin-IONPs and 46.33% in the cells treated with cisplatin-IONPs-chitosan after 24 h (Fig. 3) and also showed that the rate of necrotic cells treated with cisplatin was … portsmouth wedding

Requirement for ERK Activation in Cisplatin-induced …

IJMS Free Full-Text Multifaceted Mechanisms of …

WebWe previously described that blocking ALDH activity enhances the induction of apoptosis, especially in ovarian cancer stem cells treated with chemotherapeutic agents. ... WebMar 1, 2006 · The chloride ions in cisplatin are particularly important in its action as a drug. They are relatively stable when the drug is outside the cell, where the chloride concentration is normally high. But when the drug gets inside the cell and the chloride concentration drops, cisplatin loses its two chlorides, and they are replaced by water … oracle dba training torontoWebAug 16, 2002 · Induction of apoptosis with MMC in the human breast cancer cell line MCF-7. To determine the cytotoxic effect of various anticancer drugs with different mechanisms of action in MCF-7 cells, the ... oracle dba syllabus pdf

"WebOct 13, 2024 · Cisplatin is mainly cleared by the kidney through glomerular filtration and tubular excretion, resulting in a higher concentration of this drug in the kidney than in … " - Cisplatin apoptosis induction concentration

Cisplatin apoptosis induction concentration

Cisplatin in cancer therapy: molecular mechanisms of action

WebA high concentration of cisplatin induces apoptosis in many tumor cell lines. Whether cisplatin induces apoptosis even in a controlled release formulation has not been … WebNov 7, 2024 · Cisplatin efficiently induced cell death in OVCAR-3, OVCAR-4, and IRGOV-1 (>60% cell death) whereas OVCAR-5, OVCAR-8, and A2780 showed <30% apoptotic cells in response to cisplatin. Based on...

Did you know?

WebWe previously described that blocking ALDH activity enhances the induction of apoptosis, especially in ovarian cancer stem cells treated with chemotherapeutic agents. ... According to Table 1, the combination of cisplatin at the concentration of 0.4 µM, which is a reduction by 51.06-fold from 22 µM, with paclitaxel at 0.03 µM, ... WebApr 25, 2014 · The dosage of cisplatin determines whether the cells die by necrosis or apoptosis [30]. In cell culture studies, high concentrations of cisplatin cause necrotic cell death but lower concentrations lead to apoptosis. However,in vivo, cisplatin induces both necrosis and apoptosis [31].

WebIn contrast, in the spheroids treated with cisplatin (33 μM) an evident increase in the activity of these caspases is observed up to 1500 RLUs, indicating induction of apoptosis. To confirm the lack of apoptosis activation, the activity of initiator caspases 8 and 9 was measured through the effect of specific inhibitors on cell viability of ... WebOct 22, 2024 · Cisplatin is an alkylating agent which induces DNA damage through the formation of cisplatin-DNA adducts, leading to cell cycle arrest and apoptosis. In the …

WebCisplatin-induced testicular damage is a major obstacle in the application of cisplatin as chemotherapeutic agent. However, it remains as one of the most widel 掌桥科研一站式科研服务平台

WebFeb 17, 2024 · It came out that when the concentration of cisplatin was below 2 mg/L, the cell activity was basically above 90%, so the effect of cisplatin on cell activity was small at this time. However, when the concentration of cisplatin was above 2 mg/L, the cell activity was inversely proportional to the concentration of cisplatin.

WebApr 28, 2011 · For all cell lines, it is true that concentrations of cisplatin up to 30μM caused mainly a mode of cell death with clear morphological and biochemical characteristics of apoptosis, whereas the commonest phenotype observed with concentrations equal or higher than 300μM had clearly lost many of these characteristics and more closely … portsmouth webcams tugboatsWebApr 14, 2024 · Among the extracellular vesicles, apoptotic bodies (ABs) are only formed during the apoptosis and perform a relevant role in the pathogenesis of different … portsmouth west high school athleticsWebMay 3, 2024 · MTC induces apoptosis in ovarian cancer cells. (a) Photomicrographs of ES-2 cells treated with MTC for 24 h showed the concentration-dependent effect on cytotoxicity. Immunofluorescent staining to identify cleaved-PARP was performed to represent caspase activity. oracle dba scripts all in oracleWebJan 1, 2007 · Cisplatin is a broad-spectrum anticancer drug that is also widely used in experimental studies on DNA damage-induced apoptosis. Induction of apoptosis … portsmouth wellbeing serviceWebSep 1, 2024 · CDDP had differential effect on proteins expression and induced apoptosis in GC cells. a Western blot shows the effect of CDDP on protein expression in AGS and NCI - N87 cell lines. The expression of tumor suppressor protein 53 increased with CDDP concentration, whilst Akt and NFkB expressions reduced, notice the cleaved PARP … oracle dba from scratchWebCell clones were stained by Giemsa; (c) Induction of apoptosis and necrosis was analysed in untreated LTTs and those treated with IC 50 concentration of cisplatin, IC 50 … oracle dba training in marylandWebAbstract Cisplatin (DDP) is a common therapeutic option for non-small cell lung carcinoma (NSCLC). However, some patients fail to respond to the DDP chemotherapy. ... adenocarcinoma promotes proliferation via the accumulation of β-catenin 17 and regulates lung tumorigenesis through cell apoptosis and autophagy pathways. 16 USP14 is critical ... oracle dba jobs in michigan